We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy.
- Authors
Benjamin Rappaz; Elena Cano; Tristan Colomb; Jonas Ku¨hn; Christian Depeursinge; Viesturs Simanis; Pierre J. Magistretti; Pierre Marquet
- Abstract
Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.
- Subjects
MICROSCOPICAL technique; CELL cycle; FISSION (Asexual reproduction); DIGITAL technology; YEAST; QUANTITATIVE research; MASS (Physics); HIGH throughput screening (Drug development)
- Publication
Journal of Biomedical Optics, 2009, Vol 14, Issue 3, p034049
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.3147385