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- Title
An insight into the evolution of introns in the <italic>gyrase A</italic> gene of plants.
- Authors
Manna, Mrinalini; Fartyal, Dhirendra; Achary, V. Mohan M.; Agarwal, Aakrati; Reddy, Malireddy K.
- Abstract
DNA gyrase is a type II topoisomerase essential for replication and transcription in prokaryotes and eukaryotic cell organelles. The functional gyrase enzyme is an A2B2 tetramer encoded by the <italic>gyrA</italic> and <italic>gyrB</italic> genes. Most of the eukaryotic <italic>gyrase A</italic> genes possess introns while they are intron-less in prokaryotes. In the present study, we found out the evolutionary passage of intron development in <italic>gyrase A</italic> gene with the help of bioinformatics approaches. All the plant <italic>gyrase A</italic> genes studied by us were found to be a part of the nuclear genome, and their respective proteins were targeted to the organelles. Except the green alga <italic>Bathycoccus prasinos</italic>, these genes contained introns, and the positions of the homologous introns were found to be highly conserved in diverse plant lineages despite having variation in their nucleotide sequence compositions and lengths. However, in red, brown, and green algae: <italic>Chlorella variabilis</italic> and <italic>Chlamydomonas reinhardtii</italic>, homologous intron positions were not conserved, which might be due to the independent acquisition of introns. The study makes it amply evident that the introns appeared in the gene following endosymbiotic gene transfer of the <italic>gyrase A</italic> to the nuclear genome of an ancestral green plant. The land plants appear to have acquired intron-bearing <italic>gyrase A</italic> gene from a common ancestral green algae and subsequently lesser re-arrangement of introns at homologous positions resulted in their positional conservation. However, the introns which are known to be under lesser selection pressure evolved differently in various plant species in terms of base composition and lengths.
- Subjects
INTRONS; DNA topoisomerase II; DNA topoisomerases; EUKARYOTIC cells; PROKARYOTES
- Publication
Plant Systematics & Evolution, 2018, Vol 304, Issue 4, p521
- ISSN
0378-2697
- Publication type
Article
- DOI
10.1007/s00606-018-1503-6