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- Title
Paternal low protein diet perturbs inter-generational metabolic homeostasis in a tissue-specific manner in mice.
- Authors
Morgan, Hannah L.; Furse, Samuel; Dias, Irundika H. K.; Shabir, Kiran; Castellanos, Marcos; Khan, Iqbal; May, Sean T.; Holmes, Nadine; Carlile, Matthew; Sang, Fei; Wright, Victoria; Koulman, Albert; Watkins, Adam J.
- Abstract
The underlying mechanisms driving paternally-programmed metabolic disease in offspring remain poorly defined. We fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. Using artificial insemination, in combination with vasectomised male mating, we generated offspring using either NPD or LPD sperm but in the presence of NPD or LPD seminal plasma. Offspring from either LPD sperm or seminal fluid display elevated body weight and tissue dyslipidaemia from just 3 weeks of age. These changes become more pronounced in adulthood, occurring in conjunction with altered hepatic metabolic and inflammatory pathway gene expression. Second generation offspring also display differential tissue lipid abundance, with profiles similar to those of first generation adults. These findings demonstrate that offspring metabolic homeostasis is perturbed in response to a suboptimal paternal diet with the effects still evident within a second generation. Poor paternal diet leads to changes in offspring tissue lipid abundance that is still evident in a second generation.
- Subjects
LOW-protein diet; PATERNAL age effect; HOMEOSTASIS; DIETARY proteins; ARTIFICIAL insemination; LABORATORY mice
- Publication
Communications Biology, 2022, Vol 5, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-022-03914-8