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- Title
Proteasome Subunits mRNA Expressions Correlate With Male BMI: Implications for a Role in Obesity.
- Authors
Kozue Sakamoto; Youichi Sato; Toshikatsu Shinka; Masako Sei; Isoko Nomura; Mayumi Umeno; Ewis, Ashraf A.; Nakahori, Yutaka
- Abstract
Obesity as well as its associated chronic diseases and adverse health consequences such as type 2 diabetes mellitus, dyslipidemia, hypertension, and coronary artery disease are afflicting middle-aged adults and an ever greater number of children globally. We planned to investigate new obesity-related factors using proteomics approaches in a randomly selected three high and three low BMI samples of Epstein–Barr-transformed B (EBV-B) lymphoblastoid cell lines prepared from two groups of young Japanese men with different BMI. To search novel obesity-related factors, comparisons of protein expressions between high and low BMI groups were carried out by two-dimensional gel electrophoresis (2-DE). Gene transcripts of proteasome subunits found out from 2-DE were further determined by quantitative real-time PCR. Results from proteomics approach showed that the expression of proteasome α subunit type 5 (PSMA5) was significantly lower in the high BMI male group than in those with low BMI (P < 0.05). To validate these results, we expanded the study to include 20 more men and used real-time PCR to quantify the mRNA expression level in their EBV-B cells. Both PSMA5 and PSMA2 of EBV-B cells showed negative correlation with BMI. Furthermore, the mRNA levels measured in the peripheral blood B lymphocytes for many proteasome subunits in 75 healthy men and women showed significant negative correlation with BMI in healthy men. Our findings suggest that proteasome expression may play a key role in obesity.Obesity (2009) 17 5, 1044–1049. doi:10.1038/oby.2008.612
- Subjects
MESSENGER RNA; GENE expression; BODY mass index; ANTHROPOMETRY; OBESITY
- Publication
Obesity (19307381), 2009, Vol 17, Issue 5, p1044
- ISSN
1930-7381
- Publication type
Article
- DOI
10.1038/oby.2008.612