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- Title
Investigation of the fate of Sry-expressing cells using an in vivo Cre/ loxP system.
- Authors
Ito, Masanori; Yokouchi, Kou; Yoshida, Kazuhisa; Kano, Kiyoshi; Naito, Kunihiko; Miyazaki, Jun-Ichi; Tojo, Hideaki
- Abstract
Sry (sex-determining region on Y chromosome) is expressed in the undifferentiated, bipotential genital ridges of mammalian XY fetuses. The expression of Sry initiates testis development, but the lineage of Sry-expressing cells is unclear. In this study, double-transgenic mice were analyzed using the Cre/ loxP system. Cre under the control of the Sry promoter was expressed in the fetal gonads of transgenic mice similarly to endogenous Sry. The Sry/Cre-transgenic mice were crossed with CAG(cytomegalovirus immediate-early enhancer, chicken β- actin promoter and fusion intron of chicken β- actin and rabbit β- globin)/ loxP/ CAT/ loxP/ LacZ-transgenic mice, in which the transgene expressed β-galactosidase after a Cre-mediated recombination event. Sertoli cells, germ cells of testes and granulosa cells of ovaries of double-transgenic mice stained positive with X-gal. Cre expression was detected in germ cells and peritubular/Sertoli cells in adult testes. It is not clear whether β-galactosidase expression in the Sertoli cells of the testes occurred as a result of Cre expression in the adult or in the fetal gonads. These analyses indicate that cells expressing Sry-inducing factors in female fetal gonads become granulosa cells.
- Subjects
SEX chromosomes; Y chromosome; SPERMATOGENESIS; SERTOLI cells; LABORATORY mice; FETAL development; DEVELOPMENTAL biology
- Publication
Development, Growth & Differentiation, 2006, Vol 48, Issue 1, p41
- ISSN
0012-1592
- Publication type
Article
- DOI
10.1111/j.1440-169X.2006.00844.x