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- Title
Self-Mixing Interferometry-Based Micro Flow Cytometry System for Label-Free Cells Classification.
- Authors
Yu Zhao; Xuefei Shen; Menglei Zhang; Jingwen Yu; Jintao Li; Xiuhong Wang; Julien Perchoux; da Costa Moreira, Raul; Tao Chen
- Abstract
In this paper, we present a novel optical microfluidic cytometry scheme for label-free detection of cells that is based on the self-mixing interferometry (SMI) technique. This device enables simple, fast and accurate detection of the individual cell characteristics and efficient cell type classification. We also propose a novel parameter to classify the cell or particle size. Artificial polystyrene beads and human living cells were measured using this system, and the SMI signal properties were statistically evaluated. The capability of the proposed cytometer for cell type discrimination and size classification has been validated by the measurement results. Our study can provide a very simple technique for cell enumeration and classification without any extra devices such as high-speed camera, photomultiplier and spectrometer. Moreover, the fluorescence staining operation which is necessary in traditional flow cytometry methods is not required either in our system.
- Subjects
FLOW cytometry; CELL size; CELLS; CYTOMETRY; MICROFLUIDIC devices
- Publication
Applied Sciences (2076-3417), 2020, Vol 10, Issue 2, p478
- ISSN
2076-3417
- Publication type
Article
- DOI
10.3390/app10020478