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- Title
Metal-organic frameworks for precise inclusion of single-stranded DNA and transfection in immune cells.
- Authors
Shuang Peng; Binglin Bie; Yangzesheng Sun; Min Liu; Hengjiang Cong; Wentao Zhou; Yucong Xia; Heng Tang; Hexiang Deng; Xiang Zhou
- Abstract
Effective transfection of genetic molecules such as DNA usually relies on vectors that can reversibly uptake and release these molecules, and protect them from digestion by nuclease. Non-viral vectors meeting these requirements are rare due to the lack of specific interactions with DNA. Here, we design a series of four isoreticular metal-organic frameworks (Ni- IRMOF-74-II to -V) with progressively tuned pore size from 2.2 to 4.2 nm to precisely include single-stranded DNA (ssDNA, 11-53 nt), and to achieve reversible interaction between MOFs and ssDNA. The entire nucleic acid chain is completely confined inside the pores providing excellent protection, and the geometric distribution of the confined ssDNA is visualized by Xray diffraction. Two MOFs in this series exhibit excellent transfection efficiency in mammalian immune cells, 92% in the primary mouse immune cells (CD4+ T cell) and 30% in human immune cells (THP-1 cell), unrivaled by the commercialized agents (Lipo and Neofect).
- Subjects
SINGLE-stranded DNA; METAL-organic frameworks; NUCLEIC acids; GEOMETRIC distribution; T cells; CELLS
- Publication
Nature Communications, 2018, Vol 9, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-018-03650-w