We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Chromosomal mapping of tandem repeats in the Yesso Scallop, Patinopecten yessoensis (Jay, 1857), utilizing fluorescence in situ hybridization.
- Authors
Xuan Li; Zujing Yang; Huan Liao; Zhengrui Zhang; Xiaoting Huang; Zhenmin Bao
- Abstract
Construction of cytogenetic maps can provide important information for chromosome identification, chromosome evolution and genomic research. However, it hasn't been conducted in many scallop species yet. In the present study, we attempted to map 12 fosmid clones containing tandem repeats by fluorescence in situ hybridization (FISH) in the Yesso scallop Patinopecten yessoensis (Jay, 1857). The results showed 6 fosmid clones were successfully mapped and distributed in 6 different pairs of chromosomes. Three clones were respectively assigned to a pair of metacentric chromosomes, a pair of submetacentric chromosomes and a pair of telocentric chromosomes and the remaining 3 clones showed their loci on three different pairs of subtelocentric chromosomes by co-hybridization. In summary, totally 8 pairs of chromosomes of the Yesso scallop were identified by 6 fosmid clones and two rDNA probes. Furthermore, 6 tandem repeats of 5 clones were sequenced and could be developed as chromosome specific markers for the Yesso scallop. The successful localization of fosmid clones will undoubtedly facilitate the integration of linkage groups with cytogenetic map and genomic research for the Yesso scallop.
- Subjects
PATINOPECTEN; SCALLOPS; PHOTOLUMINESCENCE; IN situ hybridization; SPECIES
- Publication
Comparative Cytogenetics, 2016, Vol 10, Issue 1, p157
- ISSN
1993-0771
- Publication type
Article
- DOI
10.3897/CompCytogen.v10i1.7391