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- Title
Expression of collagenase and potential transcriptional factors c-fos and egr-1 in periodontal gingival fibroblasts.
- Authors
Trabandt, A.; Gay, R. E.; Sukhatme, V. P.; Gay, S.
- Abstract
In view of the important role of fibroblast-type collagenase in the pathogenesis of periodontal disease (PD), we investigated the expression of this metalloproteinase in primary cultures of non-stimulated fibroblasts dissected from gingival tissues of patients with generalized moderate and localized severe chronic adult PD. Enhanced hybridization signals for collagenase RNA were observed in 8/8 PD-cases when compared with equivalent RNA amounts extracted from normal fibroblasts. Since both the proto-oncogene c-fos and the "early growth response" gene egr-1 might be involved in the transcriptional regulation of the collagenase gene expression in vivo, we also compared the relative expression of both potential transcriptional factors with collagenase RNA in the same fibroblast cytoplasmic extracts. Hybridization signals indicated elevated RNA amounts for c-fos in 8/8 PD-cases and for egr-1 in 7/8 PD-cases when compared with the cells from non-inflamed tissue. In periodontitis gingival tissue specimens, immunolocalization of collagenase could be confirmed in fibroblasts, macrophages and epithelial cells in situ. Collagenase label was not widely distributed within the tissues, but concentrated at the interface between epithelium and connective tissue. The data provide the first evidence that gingival fibroblasts producing elevated levels of collagenase RNA amounts express also c-fos and egr-1 indicating a crucial role for both genes in cellular proliferation and collagenase expression in gingival and periodontal tissue destruction in vivo.
- Subjects
PERIODONTAL disease; COLLAGENASES; FIBROBLASTS; METALLOPROTEINASES; RNA; AMINO acids; BONE resorption; COMPARATIVE studies; DNA probes; GENE expression; GINGIVAL hyperplasia; GINGIVA; IMMUNOBLOTTING; IMMUNOENZYME technique; RESEARCH methodology; MEDICAL cooperation; NUCLEIC acid hybridization; ONCOGENES; PERIODONTITIS; PROTEINS; PROTEOLYTIC enzymes; RESEARCH; TRANSCRIPTION factors; DNA-binding proteins; EVALUATION research
- Publication
Journal of Oral Pathology & Medicine, 1992, Vol 21, Issue 5, p232
- ISSN
0904-2512
- Publication type
journal article
- DOI
10.1111/j.1600-0714.1992.tb00108.x