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- Title
Profile of the bovine acute-phase response following an intravenous bolus-dose lipopolysaccharide challenge.
- Authors
Carroll, Jeffery A.; Reuter, Richard R.; Chase Jr., Chadwick C.; Coleman, Samuel W.; Riley, David G.; Spiers, Donald E.; Arthington, John D.; Galyean, Michael L.
- Abstract
Our objective was to characterize further the acute-phase response following endotoxin (i.e. lipopolysaccharide; LPS) exposure in the bovine. Nine pure-bred Angus castrated males (i.e. steers; average body weight = 299 ±5 kg) were used in a randomized complete block design in environmentally controlled chambers, set at thermoneutral level, to characterize the acute physiological, endocrine, immune, and acute-phase protein responses following an i.v. bolus administration of 2.5 jig of LPS/kg body weight. One day before administration of LPS, all steers were fitted with an indwelling jugular vein catheter for serial blood collection. Blood samples were collected at 30-mm intervals from -2 h to 8 h relative to the LPS challenge (time 0), and serum was harvested and stored at -80°C until analyzed for concentrations of cortisol, pro-inflammatory cytokines, and acute-phase proteins. Indicators of thermal status (i.e. rectal temperature, ruminal temperature, respiration rate, sweat rate, and skin temperatures) were measured at 30-mm intervals from - I h to 6 h relative to the challenge. Endotoxin exposure increased (P <0.05) serum concentrations of cortisol, tumor necrosis factor-α (TNF-α), interleukin 1-β (IL-1β), IL-6, interferon-y (IFN-γ), and serum amyloid A. Respiration rate, rectal temperature, and rump skin temperature also were increased (P<0.05) following LPS administration. Endotoxin exposure dramatically decreased ear skin temperature (P = 0.002), but tended to increase (P <0.10) ruminal temperature, shoulder skin temperature, and shoulder sweat rate. Serum concentrations of acid soluble protein, a-acid glycoprotein, IL-4 and IL-2, and rump sweat rate were not altered (P>0.24) by the challenge. To our knowledge, this report is the most complete characterization of the bovine acute-phase response to a bolus-dose endotoxin challenge conducted under thermoneutral conditions and should provide foundation data for future research.
- Subjects
ENDOTOXINS; CATTLE vaccination; ACUTE phase proteins; ACUTE phase reaction; IMMUNE response; ANIMAL behavior endocrinology; HYDROCORTISONE; TUMOR necrosis factors; THERAPEUTICS
- Publication
Innate Immunity, 2009, Vol 15, Issue 2, p81
- ISSN
1753-4259
- Publication type
Article
- DOI
10.1177/1753425908099170