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- Title
Telomere attrition in heart failure: a flow-FISH longitudinal analysis of circulating monocytes.
- Authors
Teubel, Iris; Elchinova, Elena; Roura, Santiago; Fernández, Marco A.; Gálvez-Montón, Carolina; Moliner, Pedro; de Antonio, Marta; Lupón, Josep; Bayés-Genís, Antoni; Fernández, Marco A; Gálvez-Montón, Carolina; Lupón, Josep; Bayés-Genís, Antoni
- Abstract
<bold>Background: </bold>Cross-sectional investigations report shorter telomeres in patients with heart failure (HF); however, no studies describe telomere length (TL) trajectory and its relationship with HF progression. Here we aimed to investigate telomere shortening over time and its relationship to outcomes.<bold>Methods: </bold>Our study cohort included 101 ambulatory patients with HF. Blood samples were collected at baseline (n = 101) and at the 1-year follow-up (n = 54). Using flow-FISH analysis of circulating monocytes, we simultaneously measured three monocyte subsets-classical (CD14++CD16-), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)-and their respective TLs based on FITC-labeled PNA probe hybridization. The primary endpoints were all-cause death and the composite of all-cause death or HF-related hospitalization, assessed at 2.3 ± 0.6 years. All statistical analyses were executed by using the SPSS 15.0 software, and included Student's t test and ANOVA with post hoc Scheffe analysis, Pearson or Spearman rho correlation and univariate Cox regression when applicable.<bold>Results: </bold>We found high correlations between TL values of different monocyte subsets: CD14++CD16+ vs. CD14++CD16-, R = 0.95, p < 0.001; CD14++CD16+ vs. CD14+CD16++, R = 0.90, p < 0.001; and CD14++CD16- vs. CD14+CD16++, R = 0.89, p < 0.001. Mean monocyte TL exhibited significant attrition from baseline to the 1-year follow-up (11.1 ± 3.3 vs. 8.3 ± 2.1, p < 0.001). TL did not significantly differ between monocyte subsets at either sampling time-point (all p values > 0.1). Cox regression analyses did not indicate that TL or ΔTL was associated with all-cause death or the composite endpoint.<bold>Conclusions: </bold>Overall, this longitudinal study demonstrated a ~ 22% reduction of TL in monocytes from ambulatory patients with HF within 1 year. TL and ΔTL were not related to outcomes over long-term follow-up.
- Subjects
TELOMERES; HEART failure; DISEASE progression; MONOCYTES; HOSPITAL care; CELL division; COMPARATIVE studies; LONGITUDINAL method; RESEARCH methodology; MEDICAL cooperation; RESEARCH; FLUORESCENCE in situ hybridization; EVALUATION research; PROPORTIONAL hazards models
- Publication
Journal of Translational Medicine, 2018, Vol 16, p1
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/s12967-018-1412-z