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- Title
Identification of Two Distinct Function γδ TCR Complexes on the Surface of a Human T Cell Clone.
- Authors
Orsini, D. L. M.; Kooy, Y. M. C.; Struyk, L.; Ossendorp, F.; Van Den Elsen, P.; Koning, F.
- Abstract
In this study we describe the expression of two T cell receptor (TCR) γ chains on the surface of a human T cell clone isolated from the peripheral blood. Each γ chain was part of an independent and functional TCR. The dual receptor T cell clone (and all subclones derived from this clone) had stable expression of this phenotype. Immunoprecipitation studies revealed the expression of non-disulfide linked TCRs by this Vγ4+Vγ9+Vδ1+ T cell clone, which was in agreement with the finding that both Vγ gene transcripts were rearranged to Cγ2-associated joining elements. Both γ chains were derived from productive rearrangements of different (allelic) genes coding for a Vγ4+ and a Vγ9+ γ-chain, and both were coupled to a Vδ1+ δ chain. Incubation of this Vγ4+Vγ9+Vδ1+ T cell clone with TCRγ-chain-specific MoAbs rapidly induced an increase in intracellular Ca++, indicating that both γ-chains are functional. Furthermore, this clone responded to stimulation with <em>S. aureus</em> derived superantigens. We suggest therefore that exogenous (super)antigens can trigger dual receptor T cells resulting in activation of these T cells.
- Subjects
T cell receptors; CLONE cells; CELL receptors; ANTIGENS; IMMUNOLOGY
- Publication
Scandinavian Journal of Immunology, 1995, Vol 41, Issue 5, p499
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1995.tb03598.x