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- Title
B-RAF V600E mutational analysis of fine needle aspirates correlates with diagnosis of thyroid nodules
- Authors
Bentz, Brandon G.; Miller, Brian T.; Holden, Joseph A.; Rowe, Leslie R.; Bentz, Joel S.
- Abstract
Abstract: Objective: A mutation of B-type RAF kinase (B-RAF) represents the most common genetic alteration in papillary thyroid cancer (PTC), possibly signifying a more aggressive biology. Fine needle aspiration (FNA) represents the most useful initial diagnostic tool of thyroid nodules. Molecular analysis of the mutation status of B-RAF in thyroid nodule FNAs may provide guidance for treatment planning. Study Design: Cross-sectional study. Subjects and Methods: A retrospective chart review was undertaken for clinically relevant data of papillary thyroid cancer (PTC), follicular variant of PTC (FV-PTC), and nonmalignant goiters. After blinded pathologic review, histologic and cytologic samples were analyzed by LightCycler PCR (LCPCR) with allele-specific fluorescent probe melting curve analysis (FMCA) for the V600E mutation of B-RAF. Results: Of the 45 patient samples analyzed, B-RAF mutation was found to be significantly higher in papillary carcinomas when compared to follicular variant of papillary thyroid carcinomas (55.6% vs 14.3%, P = 0.05). Pathologic B-RAF mutational status significantly correlated with cytologic B-RAF mutational status (P < 0.0001), cytologic interpretation (P = 0.012), and histologic diagnosis (P = 0.011). Conclusions: Determination of B-RAF V600E mutation of thyroid nodule FNAs by LCPCR may be a useful tool to guide treatment planning. These data support investigating the utility of this molecular marker in a prospective manner.
- Subjects
PROTEIN kinases; GENETIC mutation; NEEDLE biopsy; THYROID cancer diagnosis; HISTOPATHOLOGY; MOLECULAR oncology
- Publication
Otolaryngology-Head & Neck Surgery, 2009, Vol 140, Issue 5, p709
- ISSN
0194-5998
- Publication type
Article
- DOI
10.1016/j.otohns.2009.01.007