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- Title
Cenpj Regulates Cilia Disassembly and Neurogenesis in the Developing Mouse Cortex.
- Authors
Wenyu Ding; Qian Wu; Le Sun; Na Clara Pan; Xiaoqun Wang
- Abstract
Primary cilia are microtubule-based protuberances that project from the eukaryotic cell body to sense the extracellular environment. Ciliogenesis is closely correlated to the cell cycle and defects of cilia are related to human systemic diseases such as primary ciliary dyskinesia. However, the role of ciliogenesis in cortical development remains unclear. Here, we demonstrate that Cenpj, a protein that is required for centriole biogenesis, plays a role in regulating cilium disassembly in vivo. Depletion of Cenpj in neural progenitor cells results in long cilia and abnormal cilia disassembly. Radial glial cells Cenpj depletion exhibit uncompleted cell division, reduced cell proliferation, and increased cell apoptosis in the developing mouse cerebrum cortex, leading to microcephaly. In addition, Cenpj depletion causes long and thin primary cilia and motile cilia in adult neural stem cells and reduced cell proliferation in the subventricular zone. Furthermore, we show that Cenpj regulates cilia disassembly and neurogenesis through Kif2a, a plus-end-directed motor protein. These data collected from mice of both sexes provide insights into how ciliogenesis plays roles in cortical development and how primary microcephaly is induced by Cenpj mutations in humans.
- Subjects
CILIA &; ciliary motion
- Publication
Journal of Neuroscience, 2019, Vol 39, Issue 11, p1994
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.1849-18.2018