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- Title
Induction and characterization of cutaneous lymphocyte antigen on natural killer cells.
- Authors
Tsuchiyama, Junjiro; Yoshino, Tadashi; Toba, Ken; Harada, Naoki; Nishiuchi, Ritsuo; Akagi, Tadaatsu; Furukawa, Tatsuo; Takahashi, Masuiro; Fuse, Ichiro; Aizawa, Yoshifusa; Harada, Mine
- Abstract
Summary. Cutaneous lymphocyte antigen (CLA) has been reported to be expressed mainly by memory/effector T lymphocytes infiltrating inflammatory skin lesions and cutaneous T-cell lymphoma. It has been suggested that CLA is a specific homing receptor, facilitating the T-cell migration into skin lesions, and also an indicator of the skin-homing T-cell subset. In the present study, we investigated the expression of CLA in natural killer (NK) cells defined phenotypically as surface CD3– and CD56+ cells in peripheral blood. CLA was definitely expressed on CD3– CD56+ cells at a level comparable to CD3+ cells in peripheral blood of normal Japanese volunteers. After in vitro stimulation of peripheral blood mononuclear cells with interleukin 2 (IL-2) and IL-12, there was a significant increase in the number and percentage of CLA+ NK cells but not CLA+ T cells (P < 0·01). To analyse the characteristics of CLA expressed by NK cells, we investigated a CLA+ NK-leukaemia cell line, NK-YS, established from a patient with NK leukaemia/lymphoma with skin infiltration. In the in vitro study, the CLA-expressing NK-leukaemic cell line bound to E-selectin-transfected cells and was inhibited by HECA 452 antibody or neuraminidase treatment of leukaemic cells. These findings suggest that CLA expressed by NK cells is a homing receptor for the E-selectin molecule and may explain skin infiltration by NK cells and NK lymphoma cells analogous to T cells. An NK-cell subset expressing CLA must play an important role in host defence against microorganisms and neoplasms in skin lesions.
- Subjects
LYMPHOCYTES; ANTIGENS; KILLER cells
- Publication
British Journal of Haematology, 2002, Vol 118, Issue 2, p654
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.2002.03608.x