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- Title
IL-17A and IL-17F Expression in B Lymphocytes.
- Authors
Vazquez-Tello, Alejandro; Halwani, Rabih; Li, Rui; Nadigel, Jessica; Bar-Or, Amir; Mazer, Bruce D.; Eidelman, David H.; Al-Muhsen, Saleh; Hamid, Qutayba
- Abstract
Background: Recent evidence suggests that cells other than Th-17 lymphocytes express interleukin (IL)-17A and IL-17F and contribute to the production of these cytokines in immunologically mediated diseases. B lymphocytes are known to be an important source of cytokines in chronic inflammatory diseases. We therefore investigated the potential of human B lymphocytes to produce IL-17A and IL-17F. Methods: Highly purified B cells were obtained using a multiple-step separation procedure which included rosette depletion, adherence depletion, CD3+ cell magnetic activated depletion and CD19+ magnetic activated positive cell selection. In these CD19+ B cell fractions, CD3+/CD4+ and CD14+ cells were negligible (<0.2%), and CD8 and CD161 mRNAs were undetectable. The CD19+/CD20+ B cells were stimulated with IL-4, interferon-γ, IL-6, IL-23 and transforming growth factor (TGF)-β, and the expression of IL-17A and IL-17F in response to stimulation was determined by quantitative reverse transcription (RT)-PCR, Western blot, immunocytochemistry and ELISA. Results: Evidence of expression of IL-17A and IL-17F in purified B cells was obtained using RT-PCR, flow cytometry, immunofluorescence microscopy, Western immunoblotting and ELISA. Stimulation of B cells with IL-6, IL-23 or TGF-β upregulated the expression of both IL-17A and F cytokines. Conclusions: These novel findings provide evidence that cytokine-stimulated B lymphocytes could be a significant source of IL-17A and IL-17F and support the notion that these cells actively participate in immune responses via alternative mechanisms in addition to the classic release of antibodies. Copyright © 2011 S. Karger AG, Basel
- Subjects
LYMPHOCYTES; INTERLEUKIN-17; CYTOKINES; B cells; IMMUNE response
- Publication
International Archives of Allergy & Immunology, 2012, Vol 157, Issue 4, p406
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000329527