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- Title
The Role of P-Glycoprotein in Transport of Danshensu across the Blood-Brain Barrier.
- Authors
Peng-Fei Yu; Wen-Yan Wang; Gaowa Eerdun; Tian Wang; Lei-Ming Zhang; Chong Li; Feng-Hua Fu
- Abstract
Danshensu (3-(3, 4-dihydroxyphenyl) lactic acid), a water-soluble active component isolated from the root of Salvia miltiorrhiza Bunge, is widely used for the treatment of cerebrovascular diseases. The present study aims to investigate the role of Pglycoprotein in transport of Danshensu across the blood-brain barrier. Sprague-Dawley rats were pretreated with verapamil at a dose of 20mg kg-1 (verapamil group) or the same volume of normal saline (control group). Ninety minutes later, the animals were administrated with Danshensu (15mg kg-1) by intravenous injection. At 15 min, 30 min, and 60min after Danshensu administration, the levels of Danshensu in the blood and brain were detected by high-performance liquid chromatographyelectrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The results showed that Danshensu concentrations in the brain of the rats pretreated with verapamil were significantly increased. In addition, the brain-plasma ratios of the group pretreated with verapamil weremuch higher than that of the control group. There was no difference in Danshensu level in plasma between the verapamil group and control group. The findings indicated that Danshensu can pass the blood-brain barrier, and P-glycoprotein plays an important role in Danshensu transportation in brain.
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2011, Vol 8, Issue 1, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2011/713523