We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Acrylamide‐Coumarin‐Benzaldehyde as a Turn‐on Fluorescent Probe Providing an Enhanced Water Solubility for Detection of Cysteine and Homocysteine.
- Authors
Chang, Min Jung; Joo, Jin Hui; Lee, Min Hee
- Abstract
We presented an acrylamide conjugated coumarin (1) as a fluorescent probe for detection of cysteine (Cys) and homocysteine (Hcy). Probe 1 is composed of coumarin as a fluorophore, acrylamide as a reactive site to Cys/Hcy, and benzaldehyde for improvement of the water solubility. To Cys/Hcy, the acrylamide group of 1 readily undergoes Michael addition resulting in a strong fluorescence at 420 nm through an inhibition of photo‐induced electron transfer (PET). The turn‐on fluorescence change of 1 was observed for Cys and Hcy, but not seen for other biologically abundant thiols (e.g., glutathione, H2S), reactive oxygen and nitrogen species, anions, and metal ions. Moreover, 1 can give strong fluorescence upon the addition of Cys and Hcy in wide pH range and their limits of detection for Cys and Hcy turned out to be 17.25 and 8.69 μM, respectively.
- Subjects
CYSTEINE; FLUORESCENT probes; HOMOCYSTEINE; REACTIVE nitrogen species; CHARGE exchange; SOLUBILITY
- Publication
Bulletin of the Korean Chemical Society, 2019, Vol 40, Issue 6, p539
- ISSN
0253-2964
- Publication type
Article
- DOI
10.1002/bkcs.11728