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- Title
Downregulation of G3BPs inhibits the growth, migration and invasion of human lung carcinoma H1299 cells by suppressing the Src/FAK-associated signaling pathway.
- Authors
Zhang, H; Zhang, S-h; He, H-w; Zhang, C-x; Yu, D-k; Shao, R-g
- Abstract
G3BP is a RasGAP binding protein that is overexpressed in many human cancers. We previously reported that downregulation of G3BP suppressed cell growth and induced apoptosis in HCT116 cells. Here we report that both transient and stable knockdown of G3BP suppressed the growth, migration and invasion capability of human lung carcinoma H1299 cells. Moreover, downregulation of G3BP significantly inhibited the phosphorylation of Src, FAK and ERK, and the levels of NF-κB were also markedly decreased in H1299 cells. Knockdown of G3BP also decreased the expression of matrix metalloproteinase-2 (MMP-2), MMP-9 and plasminogen activator (uPA), and in vivo data demonstrated that downregulation of G3BP markedly inhibited the growth of H1299 tumor xenografts. Together, these data revealed that knockdown of G3BP inhibited the migration and invasion of human lung carcinoma cells through the inhibition of Src, FAK, ERK and NF-κB and decreased levels of MMP-2, MMP-9 and uPA.
- Subjects
TUMOR growth; GENETIC regulation; LUNG cancer &; genetics; CELLULAR signal transduction; CANCER cell growth; GENE expression; APOPTOSIS
- Publication
Cancer Gene Therapy, 2013, Vol 20, Issue 11, p622
- ISSN
0929-1903
- Publication type
Article
- DOI
10.1038/cgt.2013.62