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- Title
Pulsatilla decoction suppresses matrix metalloproteinase-7-mediated leukocyte recruitment in dextran sulfate sodium-induced colitis mouse model.
- Authors
Lin, Ming-Kuem; Yang, Ya-Ting; Lin, Li-Jen; Yu, Wei-Hsuan; Chen, Huan-Yuan
- Abstract
Background: Intestinal inflammation is considered to be an important characteristic of ulcerative colitis (UC) and the current medical treatments for UC are usually proposed to suppress abnormal intestinal immune responses. Pulsatilla decoction (PD), a traditional Chinese medicine, is frequently used in UC treatments in Asian countries; however, the mechanism of the action of PD remains unclear. In the present study, the mechanism of the action of PD was elucidated in the dextran sulfate sodium (DSS)-induced colitis mouse model, a model to mimic UC. Methods: Murine colitis was evaluated by comparing the disease activity index score. The intestinal inflammation was examined by histology analyses. The leukocyte infiltration in the colonic tissues was examined by immunohistochemistry analyses. The cytokines level in colonic tissues was examined by Multi-Plex immunoassay. The epithelial proliferation was evaluated by histological analyses. Immunofluorescence double staining was used to examine the expression of MMP-7 in the immune cells. Results: In the DSS-induced colitis mouse model, administration of PD attenuated the intestinal inflammation, with a marked decrease in colonic infiltration of innate immune cells. Immunohistochemical analyses further showed that matrix metalloproteinase-7 (MMP-7) expressed by the infiltrating leukocytes, including neutrophils and macrophages was inhibited by PD treatment. PD increases the cytokine level of IL-6 in colonic tissues. Conclusion: PD suppresses intestinal inflammation, with a marked decrease in colonic infiltration of innate immune cells, through decreasing MMP-7 expression.
- Subjects
ULCERATIVE colitis; MEDICINAL plants; HERBAL medicine; LEUCOCYTES; SODIUM; ANIMAL experimentation; IMMUNOHISTOCHEMISTRY; LIQUID chromatography; ONE-way analysis of variance; MATRIX metalloproteinases; COMPARATIVE studies; T-test (Statistics); CELL proliferation; MASS spectrometry; DESCRIPTIVE statistics; RESEARCH funding; DATA analysis software; DEXTRAN; MICE; CHINESE medicine; THERAPEUTICS
- Publication
BMC Complementary Medicine & Therapies, 2022, Vol 22, Issue 1, p1
- ISSN
2662-7671
- Publication type
Article
- DOI
10.1186/s12906-022-03696-w