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- Title
IFITM1基因克隆及其在不同生长阶段牦牛各组织中的 表达分析.
- Authors
王海鹏; 李 娟; 王 利; 郑 姚; 张 玲
- Abstract
To identify and analyze the yak IFITM1 gene transcription and protein expression levels in five yak tissues at different growth stages, primers were designed based on the common cattle IFITM1 gene. Subsequently, the yak IFITM1 gene was amplified by PCR and the sequence was analyzed using bioinformatics software. The results showed that the cDNA sequence of the yak IFITM1gene was 546bp, of which the coding (CDS) region was 375bp, encoding 124 amino acid residues. The nucleotide sequenc of IFITM1 of yak shares the highest homology to cattle, which is 98.7%. Further, quantitative real-time RT- qPCR was used to detect the transcription level of the IFITM1 gene in various yak tissues at different growth stages. The results showed that the IFITM1 has different transcription levels in the heart, liver, spleen, lung and kidney tissues of one-day-old, fifteen-month-old and five-year-old yak. The transcription level in liver tissue is significantly higher than that in spleen, lung and kidney tissues (p< 0.05). With the increase of age, the transcription level of IFITM1 in liver tissue increased at first and then decreased. The transcriptional level of IFITM1 at fifteen-month-old was significantly higher than that at one day old (p<0.01). With the increase of age, the transcription level decreased continuously in lung tissue, decreased first and then stabilized in spleen and kidney tissue, and significantly reduced in five- year- old compared with one- day- old (p<0.01). Simultaneously, an immunohistochemical technique was used to detect the localization and expression of IFITM1 protein in the yak liver. IFITM1 protein is expressed in the yak liver in three growth stages and is located in the cell membrane, and the expression of this protein shows a trend of first decreasing and then increasing with age. This study laid the foundation for the in-depth research of IFITM1 protein function.
- Publication
Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao, 2021, Vol 43, Issue 2, p139
- ISSN
1008-0589
- Publication type
Article
- DOI
10.3969/j.issn.1008-0589.202004037