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- Title
脱氢表雄酮对高脂日粮饲喂大鼠脂肪组织 炎症及脂代谢的影响.
- Authors
姜志浩; 李龙龙; 姚瑶; 马海田
- Abstract
The present study aimed to investigate the effects of dehydroepiandrosterone(DHEA) on adipose tissue inflammation and lipid accumulation in rats fed with high fat diet, to provide a basis for studying the mechanism of DHEA regulating lipid metabolism and inflammation response.[Methods] Male Sprague-Dawley(SD) rats(three groups,10 in each group) were fed with normal diet(ND),high fat diet(HFD) and high fat diet with dehydroepiandrosterone(HFD+DHEA) for 10 weeks. After that, rat adipose tissue was collected for hematoxylin-eosin(HE) staining to analyze the sizes of adipocytes. The expression levels of genes related to inflammation and lipid metabolism were detected by qPCR.[Results] Compared with the normal diet group, the adipocytes sizes significantly increased in rats fed a high fat diet(P<0.01). The mRNA expression levels of interleukin-6(IL-6),tumor necrosis factor alpha(TNF-α),acetyl-CoA carboxylase(ACC),fatty acid synthase(FAS) and sterol regulatory element binding protein-1c(SREBP-1c) significantly increased(P<0.05),while the mRAN expression levels of carnitine palmitoyltransferase-1(CPT-1),carnitine palmitoyltransferase-2(CPT-2) and peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α) significantly decreased in rats fed a high fat diet(P<0.05). Compared with the high fat diet group, the increasing of adipocytes sizes induced by high fat diet were significantly inhibited after DHEA treatment(P<0.05);DHEA treatment also significantly decreased the expression levels of pro-inflammatory and lipogenesis-related factors, while the expression levels of lipolysis-related factors significantly increased(P<0.05).[Conclusions] Dehydroepiandrosterone treatment can alleviate the inflammatory response in adipose tissue caused by a high fat diet and reduce the lipids accumulation in adipocytes.
- Subjects
HIGH-fat diet; REDUCING diets; LIPOLYSIS; ADIPOSE tissues; INFLAMMATION; LIPID metabolism; PEROXISOME proliferator-activated receptors
- Publication
Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao, 2020, Vol 43, Issue 6, p1049
- ISSN
1000-2030
- Publication type
Article
- DOI
10.7685/jnau.201911003