We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Combined Use of Phenotypic Screening and of a Novel Commercial Assay (REALQUALITY Carba-Screen) for the Rapid Molecular Detection of Carbapenemases: A Single-Center Experience.
- Authors
Novazzi, Federica; Arcari, Gabriele; Drago Ferrante, Francesca; Boutahar, Sara; Genoni, Angelo Paolo; Carcione, Davide; Cassani, Gianluca; Gigante, Paolo; Carbotti, Mattia; Capuano, Riccardo; Pasciuta, Renée; Mancini, Nicasio
- Abstract
Carbapenem resistance is a serious public health threat, causing numerous deaths annually primarily due to healthcare-associated infections. To face this menace, surveillance programs in high-risk patients are becoming a widespread practice. Here we report the performance of the combined use of a recently approved commercial multiplex real-time PCR assay (REALQUALITY Carba-Screen kit) with conventional phenotypic screening. In this three-month study, 479 rectal swabs from 309 patients across high-risk units were evaluated by combining the two approaches. Although the molecular assay showed a higher positivity rate than phenotypic screening (7.1% vs. 5%), it should be noted that the molecular method alone would have missed eight carbapenem-resistant isolates, while using only phenotypic screening would not have detected sixteen isolates. This demonstrates the complementary strengths of each method. Our study confirms the need for a combined approach to maximize the possible clinical impact of this kind of screening, ensuring a more comprehensive detection of resistant strains.
- Subjects
MEDICAL screening; DRUG resistance in microorganisms; PHENOTYPES; PUBLIC health
- Publication
Diagnostics (2075-4418), 2024, Vol 14, Issue 15, p1599
- ISSN
2075-4418
- Publication type
Article
- DOI
10.3390/diagnostics14151599