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- Title
Reduction of Helicobacter Infection in IL-10<sup>−/–</sup> Mice is Dependent On CD4<sup>+</sup> T Cells but not on Mast Cells.
- Authors
Velin, Dominique; Bachmann, Daniel; Bouzourene, Hanifa; Michetti, Pierre
- Abstract
Background: In contrast to wild type, interleukin-10-deficient (IL-10−/–) mice are able to clear Helicobacter infection. In this study, we investigated the immune response of IL-10−/– mice leading to the reduction of Helicobacter infection. Materials and Methods: We characterized the immune responses of Helicobacter felis-infected IL-10−/– mice by studying the systemic antibody and cellular responses toward Helicobacter. We investigated the role of CD4+ T cells in the Helicobacter clearance by injecting H. felis-infected IL-10−/– mice with anti-CD4 depleting antibodies. To examine the role of mast cells in Helicobacter clearance, we constructed and infected mast cells and IL-10 double-deficient mice. Results: Reduction of Helicobacter infection in IL-10−/– mice is associated with strong humoral (fivefold higher serum antiurease antibody titers were measured in IL-10−/– in comparison to wild-type mice, p < .008) and cellular (urease-stimulated splenic CD4+ T cells isolated from infected IL-10−/– mice produce 150-fold more interferon-γ in comparison to wild-type counterparts, p < .008) immune responses directed toward Helicobacter. Depletion of CD4+ cells from Helicobacter-infected IL-10−/– mice lead to the loss of bacterial clearance (rapid urease tests are threefold higher in CD4+ depleted IL-10−/– in comparison to nondepleted IL-10−/– mice, p < .02). Mast cell IL-10−/– double-deficient mice clear H. felis infection, indicating that mast cells are unnecessary for the bacterial eradication in IL-10−/– mice. Conclusion: Taken together, these results suggest that CD4+ cells are required for Helicobacter clearance in IL-10−/– mice. This reduction of Helicobacter infection is, however, not dependent on the mast cell population.
- Subjects
HELICOBACTER pylori infections; INTERLEUKIN-10; IMMUNE response; MAST cells; T cells; CD4 antigen
- Publication
Helicobacter, 2008, Vol 13, Issue 5, p361
- ISSN
1083-4389
- Publication type
Article
- DOI
10.1111/j.1523-5378.2008.00614.x