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- Title
Effect ofH. pylorion the Expression of TRAIL, FasL and their Receptor Subtypes in Human Gastric Epithelial Cells and their Role in Apoptosis.
- Authors
Martin, Jan Hendrik; Potthoff, Andrej; Ledig, Susanne; Cornberg, Markus; Jandl, Oliver; Manns, Michael P.; Kubicka, Stefan; Flemming, Peer; Athmann, Christoph; Beilt, Winfried; Wagner, Siegfried
- Abstract
In the human stomach expression of TNF-related apoptosis inducing ligand (TRAIL) and its receptors and the modulatory role ofHelicobacter pyloriare not well described. Therefore, we investigated the effect ofH. pylorion the expression of TRAIL, FasL and their receptors (TRAIL-R1-R4, Fas) in gastric epithelial cells and examined their role in apoptosis.mRNA and protein expression of TRAIL, FasL and their receptors were analyzed in human gastric epithelial cells using RT-PCR, Western blot, and immunohistochemistry. Gastric epithelial cells were incubated with FasL, TRAIL and/orH. pylori, and effects on expression, cell viability and epithelial apoptosis were monitored. Apoptosis was analyzed by histone ELISA, DAPI staining and immunohistochemistry.TRAIL, FasL and their receptor subtypes were expressed in human gastric mucosa, gastric epithelial cell primary cultures and gastric cancer cells. TRAIL, FasL andH. pyloricaused a time- and concentration-dependent induction of DNA fragmentation in gastric cancer cells with synergistic effects. In addition,H. pyloricaused a selective up-regulation of TRAIL, TRAIL-R1 and Fas mRNA and protein expression in gastric cancer cells.Next to FasL and Fas, TRAIL and all of its receptor subtypes are expressed in the human stomach and differentially modulated byH. pylori. TRAIL, FasL andH. pylorishow complex interaction mediating apoptosis in human gastric epithelial cells. These findings might be important for the understanding of gastric epithelial cell kinetics in patients withH. pyloriinfection.
- Subjects
TUMOR necrosis factors; APOPTOSIS; EPITHELIUM; LIGANDS (Biochemistry); HELICOBACTER pylori; BACTERIA; IMMUNOHISTOCHEMISTRY; POLYMERASE chain reaction
- Publication
Helicobacter, 2004, Vol 9, Issue 5, p371
- ISSN
1083-4389
- Publication type
Article
- DOI
10.1111/j.1083-4389.2004.00269.x