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- Title
Optimization of Ultrasonic Extraction Process of Oenanthe benghalensss Polysaccharide by Orthogonal Test.
- Authors
Liufu LU; Aiyue LUO; Bingyang LIN; Zhenlian XIE; Guangyu GAN; Xiuzhen HUANG; Zhidong LEI; Shimei CHEN; Suoyi HUANG
- Abstract
[Obbectives] The research aimed to explore extraction method of Oenanthe benghalensis polysaccharide, and determine the optimal process of its ultrasonic extraction by orthogonal test. [Methods] Phenol - sulfuric acid method was used to determine polysaccharide's content. Based on single-factor experiment and orthogonal test, the influences of solid - liquid ratio (A), ultrasonic extraction temperature (B), ultrasonic power (C) and ultrasonic time (D) on extraction rate of O. benghalensis polysaccharide were studied, and the optimal procees of ultrasonic extraction was selected. [Results] The influence order of solid - liquid ratio (A), ultrasonic extraction temperature (B), ultrasonic power (C) and ultrasonic time (D) on extraction rate of O. benghalensis polysaccharide was A > D > B > C. Via the orthogonal test, it was determined that the optimal extraction condition of O. benghalensis polysaccharide was as below % solid - liqquid ratio 1:25 (g/mL), extraction temperature 6°C, extraction power 80 W, extraction time 40 min. Under the condition, extraction rate of O. benghalens is polysaccharide was 9.80%. & Conclusions] The procees could provide the reference basis for extracting and producing O. benghalens is polysaccharides.
- Subjects
WHEATEARS; POLYSACCHARIDES; PLANT extracts; TEMPERATURE effect; SOLID-liquid interfaces
- Publication
Medicinal Plant, 2018, Vol 9, Issue 5, p23
- ISSN
2152-3924
- Publication type
Article
- DOI
10.19600/j.cnki.issn2152-3924.2018.05.025