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- Title
High throughput fluorescent CE-SSCP SNP genotyping.
- Authors
Gonen, D.; Veenstra-VanderWeele, J.; Yang, Z.; Leventhal, B.L.; Cook Jr., E.H.
- Abstract
Large numbers of single nucleotide polymorphisms (SNPs) are being identified by several laboratories for the purpose of developing dense genetic maps. Single-strand conformation polymorphism (SSCP) analysis has been widely used as a method for detecting novel sequence variations in PCR products. Differences in migration of single-stranded DNA can be used not only to find mutations, but to genotype SNPs in large sample populations. Using PCR with fluorescent labeling and automated capillary electrophoresis SSCP (CE-SSCP), we have developed a panel of 15 functional candidate SNPs. With an automated single capillary instrument, relatively rapid and Iow cost CE-SSCP SNP genotyping using currently available technology is feasible for 135 000 genotypes per year. With parallel multiple array capillary electrophoresis, more genotypes per year may be attainable.
- Subjects
FLUORESCENT antibody technique; GENETIC polymorphisms
- Publication
Molecular Psychiatry, 1999, Vol 4, Issue 4, p338
- ISSN
1359-4184
- Publication type
Article
- DOI
10.1038/sj.mp.4000564