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- Title
The in vitro derivation of phenotypically mature and diverse B cells from immature spleen and bone marrow precursors.
- Authors
Roundy, Kirstin M.; Jacobson, Amanda C.; Weis, Janis J.; Weis, John H.
- Abstract
The capacity of immature B cells of the spleen and bone marrow to differentiate in vitro into cells representing mature end stage cells was investigated using B-cell activating factor belonging to the TNF family (BAFF) and Notch pathway activators. Immature splenic and bone marrow B cells were found, in the presence of both of these activators, to mature into cells with follicular mature (FM) and marginal zone (MZ) cell phenotypes. Such cells were functionally responsive to B-cell-specific activation. The derivation in vitro of cells with an MZ phenotype was more robust from CD23 populations than CD23 immature/transitional B cells, suggesting a direct immature/T1 B cell to MZ cell differentiation pathway. Transcript analysis of the in vitro-derived B-cell populations demonstrated expression profiles similar to maturing B cells in vivo. FACS-purified populations of B220CD19CD21CD23 cells from bone marrow of 2-wk-old mice gave rise to populations of CD21CD23 cells with MZ cell phenotypes as well as CD21CD23 cells with FM cell phenotypes in percentages similar to those found in vivo. These data suggest that the commitment to an MZ and FM B cell phenotype is set prior to immature B-cell release from the marrow.
- Publication
European Journal of Immunology, 2010, Vol 40, Issue 4, p1139
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200939661