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- Title
Molecular cloning and expression of amylosucrase from highly radiation-resistant Deinococcus radiopugnans.
- Authors
Kim, Myo-Deok; Seo, Dong-Ho; Jung, Jong-Hyun; Jung, Dong-Hyun; Joe, Min-Ho; Lim, Sangyong; Lee, Ju-Hoon; Park, Cheon-Seok
- Abstract
Amylosucrase (AS), a glucosyltransferase of the glycoside hydrolase 13 family, does not require a nucleotide-activated sugar as a glucosyl-donor. A gene ( drpas) of Deinococcus radiopugnans encoding a putative AS was identified and cloned for characterization. The amino acid sequence revealed that drpas exhibited 76 and 74% identities with AS genes from D. geothermalis and D. radiodurans, respectively. Recombinant DRpAS exhibited AS activities. The ratios of hydrolysis, polymerization, and isomerization reactions were 5.8:82.7:11.5 at 40°C. The DRpAS was highly thermostable and produced more polymerization products than AS from D. radiodurans although the optimum growth temperature of both strains is 30°C.
- Publication
Food Science & Biotechnology, 2014, Vol 23, Issue 6, p2007
- ISSN
1226-7708
- Publication type
Article
- DOI
10.1007/s10068-014-0273-3