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- Title
A High-Throughput Assay for siRNA-Based Circadian Screens in Human U2OS Cells.
- Authors
Vollmers, Christopher; Panda, Satchidananda; DiTacchio, Luciano
- Abstract
The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.
- Subjects
SMALL interfering RNA; GENETIC testing; MICROBIOLOGICAL assay; CIRCADIAN rhythms; BIOLUMINESCENCE assay; GENE mapping; EFFECT of chemicals on human cell culture; ATTACHMENT mechanisms (Biology)
- Publication
PLoS ONE, 2008, Vol 3, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0003457