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- Title
Defects of Insulin Action and Skeletal Muscle Glucose Metabolism in Growth Hormone-Deficient Adults Persist after 24 Months of Recombinant Human Growth Hormone Therapy.
- Authors
CHRISTOPHER, MICHAEL; FEN LEE HEW; OAKLEY, MARION; RANTZAU, CHRISTIAN; ALFORD, FRANK
- Abstract
We have previously reported that GH-deficient (GHD) adults are severely insulin resistant. In the present study, we determined the effects of 6 months (n = 7) and 24 months (long-term; n = 11) of recombinant human GH (rhGH) therapy (~0.22 IU/kg.week) on body composition and fasting biochemical (including lipid) parameters, and baseline and insulin-stimulated: 1) rates of hepatic glucose production, total glucose disposal (Rd), total glycolysis (GF) and glucose storage (GS); and 2) skeletal muscle glucose processing [using the euglycemic-hyperinsulinemic (~60 mU/L) clamp technique with tritiated glucose infusion coupled with skeletal muscle biopsies]. To allow baseline comparison, these measurements were also obtained from 10 control subjects matched to the pretreated GHD adults for age, sex, and body mass index. Long-term rhGH therapy in GHD adults induced significant improvements in fat mass, abdominal fat mass and fat free mass, and reductions in fasting cholesterol and low-density lipoprotein-cholesterol levels (P < 0.05–0.01 vs. pretreatment values). However, there was a significant increase in fasting insulin (13.1 ± 0.9 vs. 8.6 ± 1.1 mU/L; P < 0.01) and connecting peptide (0.56 ± 0.05 vs. 0.41 ± 0.06 nmol/L; P < 0.05). Although rates of baseline hepatic glucose production, GF, and GS were unchanged, the insulin-stimulated increment (Δ) in Rd, GF, and GS remained markedly attenuated in the long-term rhGH-treated GHD adults [pretreatment: ΔRd 16.6 ± 3.4, ΔGF 3.0 ± 1.2, DGS 13.6 ± 3.0 vs. 24 months of rhGH: ΔRd 17.2 ± 3.3, ΔGF 3.1 ± 0.9, DGS 14.1 6 2.5 vs. controls: ΔRd 42.6 ± 4.3, ΔGF 9.2 ± 1.9, ΔGS 35.9 ± 4.5 mmol/kg fat free masszmin; P < 0.05–0.01 vs. controls]. Additionally, there was a sustained reduction in the insulin-stimulated skeletal muscle glycogen synthase fractional velocity (pretreatment: 0.29 ± 0.03 vs. 24 months of rhGH: 0.24 ± 0.03 vs. controls: 0.48 ± 0.04; both P < 0.05 vs. controls), which was accompanied by a sustained 44% decrease in baseline glycogen content and a 70% increase in baseline im glucose concentrations in the presence of low-to-normal glucose 6-phosphate levels and persisting euglycemia. Stepwise regression analysis revealed that body weight and fasting free fatty acid and high-density lipoprotein (HDL)-cholesterol accounted for 82% of the variance in the insulin sensitivity index in long-term rhGH-treated adults, and that the 24-month fasting insulin-like growth factor 1 was a negative predictor of the change in insulin sensitivity (r = -0.82; P < 0.01). In conclusion, despite improvements in body composition and lipid profiles, the severe defects of in vivo insulin sensitivity and skeletal muscle intracellular glucose phosphorylation and glycogen synthase activity, which are associated with modestly elevated insulin-like growth factor 1 levels, normal free fatty acid levels, and the development of hyperinsulinemia, persist with long-term rhGH therapy.
- Publication
Journal of Clinical Endocrinology & Metabolism, 1998, Vol 83, Issue 5, p1668
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/jc.83.5.1668