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- Title
Haploid plant regeneration from hardy kiwifruit (Actinidia arguta Planch.) anther culture.
- Authors
Wang, Guang-Fu; Qin, Hong-Yan; Sun, Dan; Fan, Shu-Tian; Yang, Yi-Ming; Wang, Zhen-Xing; Xu, Pei-Llei; Zhao, Ying; Liu, Ying-Xue; Ai, Jun
- Abstract
Haploids are important for studying plant breeding and genome sequencing. Regeneration from in vitro anther culture under aseptic conditions has achieved success in many higher plants. This study focused on anther culture of hardy kiwifruit (Actinidia arguta Planch.) to obtain haploid or double haploid plants that originated from microspores. Using the anthers of hardy kiwifruit as the test materials, we report the relationship between the microspore development stage and the bud morphological characteristics, some factors influenced the callus induction and the differentiation of the adventitious buds. The results showed that microspore development correlated with the bud diameter and morphological characteristics during development. The microspore development stage, pretreatment at low temperature (4 °C), basic medium, plant growth regulators and genotype all affect hardy kiwifruit anther callus induction, and the type and concentration of plant growth regulators significantly affect the regeneration of adventitious buds. The ploidy of 119 regenerated plants was verified using flow cytometry. The results showed that the ploidy of the plantlets obtained from the vitro anther culture of hardy kiwifruit was complex, being 3.36% haploid, 56.3% tetraploid, 4.20% hexaploid, and 36.13% octoploid.
- Subjects
KIWIFRUIT; PLANT growth
- Publication
Plant Cell, Tissue & Organ Culture, 2018, Vol 134, Issue 1, p15
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-018-1396-7