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- Title
A simple protocol for transfecting human mesenchymal stem cells.
- Authors
de Carvalho, Talita Giacomet; Pellenz, Felipe Matheus; Laureano, Alvaro; da Rocha Silla, Lucia Mariano; Giugliani, Roberto; Baldo, Guilherme; Matte, Ursula
- Abstract
Objectives and results: Mesenchymal stromal cells (MSCs) are potential targets for cell and gene therapy-based approaches against a variety of different diseases. The MSCs from bone marrow are a promising target population as they are capable of differentiating along multiple lineages and have significant expansion capability. These characteristics make them strong candidates for delivering genes and restoring organ systems function. However, as other primary cells, MSCs are difficult to transfect. In order to standardize a simple protocol for transfection of MSCs, we conducted a series of experiments and achieved a protocol that does not require the use of viral particles or specific expensive equipment.Conclusion: MSCs transfection at early passages using a ratio lipid/DNA of 3.0 µL/µg with Lipofectamine 3000® yields good transfection efficiencies for human MSCs (up to 26%) and is rapid, simple, and safe.
- Subjects
MESENCHYMAL stem cells; GENE transfection; GENE therapy; GENE targeting; GENE delivery techniques
- Publication
Biotechnology Letters, 2018, Vol 40, Issue 3, p617
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-018-2505-8