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- Title
Enzymatic esterification of free fatty acids in vegetable oils utilizing different immobilized lipases.
- Authors
Haar, Daniela; Stäbler, Andreas; Wichmann, Rolf; Schweiggert-Weisz, Ute
- Abstract
Different immobilized lipases were screened for their ability to esterify free fatty acids (FFA) with monoacylglycerol (MAG) as acyl-group acceptor. A lipase from Rhizomucor miehei (Lipozyme RMIM) was the most suitable for lipase-catalyzed de-acidification-a promising alternative to conventional neutralization. A reduction of the FFA content to 0.6 % (w/w) was achieved by applying a substrate with an initial FFA-content of 6 % (w/w), the reaction at 50 °C for 22 h as well as the stepwise addition of a quadruple stoichiometric amount of MAG.
- Subjects
VEGETABLE oil analysis; FREE fatty acids; ESTERIFICATION; ENZYMATIC analysis; LIPASES; MONOGLYCERIDES; STOICHIOMETRY
- Publication
Biotechnology Letters, 2015, Vol 37, Issue 1, p169
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1668-1