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- Title
Transcripts and sequence elements suggest differential promoter usage within the ycf3-psaAB gene cluster on mustard (Sinapis alba L.) chloroplast DNA.
- Authors
Summer, Holger; Pfannschmidt, Thomas; Link, Gerhard
- Abstract
The mustard chloroplast DNA region spanning the ycf3 gene and part of the psaAB operon was investigated. The ycf3 gene reveals two class-II introns that are removed during processing to give a mature 0.7-kb transcript, but no RNA editing seems to be involved. RNase protection and RT-PCR experiments suggest cotranscription of ycf3 with the downstream psaA gene, possibly from a NEP promoter upstream of ycf3, whereas distinct ycf3 and psaA transcripts are each initiated from PEP promoters. This situation is reminiscent of that for the trnK-psbA gene region. The implications for light-regulated versus light-independent expression of photosystem core-protein genes are discussed.
- Subjects
MUSTARD; CHLOROPLASTS; GENETIC transcription; NUCLEIC acids; GENES; INTRONS
- Publication
Current Genetics, 2000, Vol 37, Issue 1, p45
- ISSN
0172-8083
- Publication type
Article
- DOI
10.1007/s002940050007