We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Regeneration of tracheal neotissue in partially decellularized scaffolds.
- Authors
Tan, Zheng Hong; Dharmadhikari, Sayali; Liu, Lumei; Yu, Jane; Shontz, Kimberly M.; Stack, Jacob T.; Breuer, Christopher K.; Reynolds, Susan D.; Chiang, Tendy
- Abstract
Extensive tracheal injury or disease can be life-threatening but there is currently no standard of care. Regenerative medicine offers a potential solution to long-segment tracheal defects through the creation of scaffolds that support the generation of healthy neotissue. We developed decellularized tracheal grafts (PDTG) by removing the cells of the epithelium and lamina propria while preserving donor cartilage. We previously demonstrated that PDTG support regeneration of host-derived neotissue. Here, we use a combination of microsurgical, immunofluorescent, and transcriptomic approaches to compare PDTG neotissue with the native airway and surgical controls. We report that PDTG neotissue is composed of native tracheal cell types and that the neoepithelium and microvasculature persisted for at least 6 months. Vascular perfusion of PDTG was established within 2 weeks and the graft recruited multipotential airway stem cells that exhibit normal proliferation and differentiation. Hence, PDTG neotissue recapitulates the structure and function of the host trachea and has the potential to regenerate.
- Subjects
REGENERATIVE medicine; STEM cells; PERFUSION; AIRWAY (Anatomy); TRACHEA
- Publication
NPJ Regenerative Medicine, 2023, Vol 8, Issue 1, p1
- ISSN
2057-3995
- Publication type
Article
- DOI
10.1038/s41536-023-00312-4