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- Title
Bone marrow mesenchymal stem cells suppressing activation of allogeneic cytokine-induced killer/natural killer cells either by direct or indirect interaction.
- Authors
Li, Yang; Qu, Yu H.; Wu, Yan F.; Liu, Ling; Lin, Xiang H.; Huang, Ke; Wei, Jing
- Abstract
Bone marrow mesenchymal stem cells (MSC) were recently found to be associated with some special immunological characteristics, the immunoregulatory effect of MSC was dose-dependent. Low amount of MSC was associated with mild immunosuppression or even immune activation, while the high amount of that was associated with significant immunosuppressive effect. In this study, by using a transwell system, we explored the effect of MSC on the cell cycle, apoptosis rate and the expression of CD69, an activation marker, on the allogeneic cord blood derived cytokine-induced killer(CIK)/natural killer(NK) cells. The results showed that either by transwell or mixed cell-cell co-culture, the MSC can effect CIK/NK cells on the cell cycle, such as arrested in the G0/G1 phase, diminished the ratio of cells in S, G2/M phase, and increased the apoptosis of them. MSC can also depress the expression of CD69 on these killer cells, as well as increased the ratio of CD4+CD25+CD127low T regulatory (Treg) cells in the CIK/NK cell culture system. We draw conclusions that either by transwell or mixed co-culture, the MSC can suppress activation of allogeneic CB-CIK/NK cells in a dose-dependent manner.
- Subjects
MESENCHYMAL stem cells; CYTOKINES; KILLER cells; IMMUNOMODULATORS; IMMUNOSUPPRESSION; GENE expression
- Publication
Cell Biology International, 2015, Vol 39, Issue 4, p435
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.10404