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- Title
A procedure for obtaining erythrocytes from larval fish for cytological study and a description of larval blood of red hake, Urophycis chuss (Walbaum) and Atlantic mackerel, Scomber scombrus (Linnaeus).
- Authors
Perry, D. M.
- Abstract
means of obtaining erythrocytes from larval fish and the blood of larvae of two fish species are described. Erythrocytes were obtained for cytological preparations from larvae of Urophycis chuss (Walbaum) and Scomber scombrus (Linnaeus) as small as 2.0 mm. Heart and gills were dissected from the larvae, stained, and flattened into a monolayer on a microscope slide. Mature erythrocytes were the only blood cell type observed in either heart or gill preparations, and these appeared to be the same as in the adult fish. Mature erythrocytes can be isolated from formalinfixed larvae, even as sorted from plankton, and prepared for cytological and cytogenetic study and for blood cell counts. Knowledge gained from the study of erythrocytes from larval teleosts can be important in assessing the normality and health of these early-life stages of fish in either laboratory assays or field studies. The method described is applicable to other fish species, even after years of storage in formalin fixative.
- Publication
Journal of Fish Biology, 1987, Vol 30, Issue 6, p743
- ISSN
0022-1112
- Publication type
Article
- DOI
10.1111/j.1095-8649.1987.tb05803.x