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- Title
Whole-Genome Sequencing and Comparative Genomic Analysis of Antimicrobial Producing Streptococcus lutetiensis from the Rumen.
- Authors
de Oliveira, Isabela Maria Fernandes; Godoy-Santos, Fernanda; Oyama, Linda Boniface; Moreira, Sofia Magalhães; Dias, Rodrigo Gonçalves; Huws, Sharon Ann; Creevey, Christopher J.; Mantovani, Hilário Cuquetto
- Abstract
Antimicrobial peptides (AMPs) can efficiently control different microbial pathogens and show the potential to be applied in clinical practice and livestock production. In this work, the aim was to isolate AMP-producing ruminal streptococci and to characterize their genetic features through whole-genome sequencing. We cultured 463 bacterial isolates from the rumen of Nelore bulls, 81 of which were phenotypically classified as being Streptococcaceae. Five isolates with broad-range activity were genome sequenced and confirmed as being Streptococcus lutetiensis. The genetic features linked to their antimicrobial activity or adaptation to the rumen environment were characterized through comparative genomics. The genome of S. lutetiensis UFV80 harbored a putative CRISPR-Cas9 system (Type IIA). Computational tools were used to discover novel biosynthetic clusters linked to the production of bacteriocins. All bacterial genomes harbored genetic clusters related to the biosynthesis of class I and class II bacteriocins. SDS-PAGE confirmed the results obtained in silico and demonstrated that the class II bacteriocins predicted in the genomes of three S. lutetiensis strains had identical molecular mass (5197 Da). These results demonstrate that ruminal bacteria of the Streptococcus bovis/equinus complex represent a promising source of novel antimicrobial peptides.
- Subjects
GENOMICS; NUCLEOTIDE sequencing; STREPTOCOCCUS; ANTIMICROBIAL peptides; BACTERIAL genomes; GENE clusters; STREPTOCOCCUS thermophilus
- Publication
Microorganisms, 2022, Vol 10, Issue 3, p551
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms10030551