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- Title
Culture system for extensive production of CD19+IgM+ cells by human cord blood CD34+ progenitors.
- Authors
Ohkawara, J-I; Ikebuchi, K; Fujihara, M; Sato, N; Hirayama, F; Yamaguchi, M; Mori, K J; Sekiguchi, S
- Abstract
We established a co-culture system with a monolayer of the murine bone marrow (BM) stroma cell line, MS-5, in which human cord blood CD34+ cells differentiated to CD19+ cells. The addition of stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) highly enhanced the production of CD19+ cells. The expansion of the cell numbers was over 10(3)-fold. Furthermore, a significant proportion (<45%) of the cells expressed surface IgM (sIgM) after 5 weeks of co-culture. CD34+CD19- cells also showed a similar development of CD19+ cells and CD19+sigM+ cells. Filter separation of MS-5 cells and CD34+ cells did not inhibit the growth of CD19+ cells. However, when further purified CD34+CD19-CD13- CD33- cells were cultured in the presence of MS-5 cells with or without a separation filter, CD19+ cells did not appear in the non-contact setting. This result suggested that the highly purified CD34+CD19-CD13-CD33- progenitors require the cell-cell contact for the development of CD19+ cells, whereas other CD34+ fractions contain progenitors that do not require the contact. This co-culture system should be useful for the study of early human B-lymphopoiesis.
- Subjects
BONE marrow; BLOOD cells; IMMUNOGLOBULINS; B cells; GRANULOCYTE-colony stimulating factor; ANIMAL experimentation; RESEARCH methodology; HEMATOPOIETIC growth factors; TISSUE culture; CORD blood; CELL communication; HEMATOPOIETIC stem cells; CONNECTIVE tissue cells; CELL lines; ANTIGENS; MICE; BIOTECHNOLOGY
- Publication
Leukemia (08876924), 1998, Vol 12, Issue 5, p764
- ISSN
0887-6924
- Publication type
journal article
- DOI
10.1038/sj.leu.2401004