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- Title
Servikal Örneklerde Human Papillomavirüs Genotiplendirme ve Sitopatoloji Sonuçlarının Değerlendirilmesi.
- Authors
Yerlikaya, Hacer; Orak, Filiz; Aral, Murat; Yalçınkaya, Kezban Tülay; Koçarslan, Sezen; Güzin, Kadir; Doğaner, Adem
- Abstract
Aim: The aim of this study was to investigate human papillomavirus DNA positivity in cervical samples taken from sexually active female patients; Determination of human papillomavirus genotypes and comparison with Papanicolaou smear and colposcopic biopsy results. Methods: In this study sexually active female patients aged 18 years and over who applied to the obstetrics and gynecology clinic of a tertiary hospital between November 2020 and October 2021 were included. In vitro real-time HPV PCR method was used for the qualitative detection of human papillomavirus DNA from cervical specimens. Cervical specimens, which were sent to the pathology laboratory and stained according to Papanicolaou cytology protocol, and colposcopic biopsy specimens stained with hematoxylin-eosin were evaluated by an experienced pathologist. Results: One of the human papillomavirus genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 67 and 68 was isolated in 19 (4.9%) of 383 female patients included in the study. human papillomavirus 16 was detected in five (26.3%) patients, human papillomavirus 18 was detected in one (5.2%) patient, and one of 13 other high-risk types was detected in the remaining 13 (68.4%) patients. The mean age of women with low-risk human papillomavirus genotype was 43.41±9.90 years, and the mean age of women with human papillomavirus high-risk genotype was 41.79±8.70 years. According to the Bethesda 14 classification, normal cytology was detected in 354 patients, ASC-US in 19 (5%), ASC-H in two (0.5%), LSIL in two (0.5%), HSIL in two (0.5%), AGC in 3 (0.8%) and one patient (0.3%) had adenocarcinoma in situ. While AGC was detected in 1 (20%) of 5 patients with human papillomavirus genotype 16, ASC-H was detected in 1 (20%) patient, 3 (60%) patients were evaluated as normal. No pathology was found in the cytology examination of the patient with human papillomavirus genotype 18. In the cytological examination of other high-risk human papillomavirus genotypes isolated from 13 patients; ASCUS results were obtained in 3 (23%) patients, HSIL in 1 (7.6%) and normal results in 9 (69.2%) patients. In the biopsy examination of patients with normal cytology findings and HPV 16 positive; CIN 1 and CIN 2 were detected in one patient, CIN 3 in one HPV 18 positive patient, and squamous cell carcinoma in one of the other HR-HPV patients. Conclusion: In cancer screening and patient follow-up, cytological evaluations should be evaluated together with molecular studies. These studies will also contribute to the acquisition of regional epidemiological data.
- Publication
Dicle Medical Journal / Dicle Tip Dergisi, 2023, Vol 50, Issue 2, p235
- ISSN
1300-2945
- Publication type
Article
- DOI
10.5798/dicletip.1313386