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- Title
A Sensitive Chemiluminescence Enzyme Immunoassay for Screening Human Parvovirus B19 Antigens.
- Authors
Ikeda, H.; Niwa, H.; Kawabata, K.; Uchida, Y.; Yamamoto, K.; Sakata, H.; Miyazaki, T.; Ihara, H.; Fujiwara, Y.; Sato, S.; Kato, T.
- Abstract
Background: Human parvovirus B19 screening is a way to eliminate (or reduce) B19 contamination in blood product, since inactivation of this virus is difficult. However, except the receptor-mediated assays, no B19 screening method has been available. We developed a sensitive chemiluminescence enzyme immunoassay (CLEIA) for screening B19 antigens. Materials and methods: A newly developed B19 CLEIA is a 2-step sandwich assay using anti-B19 monoclonal antibodies with different specificities. B19 antigens in the samples were bound to solid phase-fixed anti-B19 monoclonal antibodies and detected by ALP-labeled anti-B19. B19 DNA in the samples was assayed by nested PCR using NS region sequences. B19 DNA-positive panels (n=127) were divided into 4 groups according to their anti-B19 IgG/IgM antibody reactivities. Results: CLEIA positive rates of the panels with B19 DNA concentration of ³10[sup 6], 10[sup 4-5], and <10[sup 4] copies/20µL were 100% (74/74), 86% (19/22), and 13% (4/31), respectively. Of 59 group A (anti-B19 negative), 23 group B (IgM-antiB19 positive), 35 group C (IgM/IgG-anti-B19 positive) and 10 group D (IgGanti-B19 positive) panels, 100%, 100%, 43% and 0% were CLEIA-positive, respectively. Of 1,000 random donor samples, only 1 was CLEIA-positive but B19 DNA-negative. Conclusions: The newly developed B19 CLEIA was able to detect most of B19-positive blood in early post infection period with ³10[sup 4] DNA copies/20µL and would be an effective screening method to reduce B19 contamination in pool plasma.
- Subjects
ANTIGENS; BLOOD products; BLOOD testing; CHEMILUMINESCENCE immunoassay; ENZYME-linked immunosorbent assay
- Publication
Transfusion, 2001, Vol 41, p86S
- ISSN
0041-1132
- Publication type
Article