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- Title
Processing of polycistronic guide RNAs is associated with RNA editing complexes in Trypanosoma brucei.
- Authors
Grams, Jayleen; McManus, Michael T.; Hajduk, Stephen L.
- Abstract
In kinetoplastid mitochondrial mRNA editing, posttranscriptional insertion or deletion of uridines is templated by guide RNAs (gRNAs). Pre-mRNAs are encoded by maxicircles, while gRNAs are encoded by both maxicircles and minicircles. We have investigated minicircle transcription and the processing of gRNAs in Trypanosoma brucei. We find that mini-circles are transcribed polycistronically and that transcripts are accurately processed by an ∼19S complex. This gRNA processing activity co-purifies with RNA editing complexes, and both remain associated in 19S complexes. Furthermore, we show that RNA editing complexes associate preferentially with a polycistronic gRNA over non-processed RNAs. We propose that the ∼19S complexes initially described as RNA editing complex I are gRNA processing complexes that cleave polycistronic gRNA transcripts into monocistrons.
- Subjects
TRYPANOSOMA brucei; RNA; GENETIC transcription; RNA editing; GENETIC regulation; MOLECULAR biology; BIOCHEMISTRY
- Publication
EMBO Journal, 2000, Vol 19, Issue 20, p5525
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/19.20.5525