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- Title
Nongenetic method for purifying stem cell–derived cardiomyocytes.
- Authors
Hattori, Fumiyuki; Chen, Hao; Yamashita, Hiromi; Tohyama, Shugo; Satoh, Yu-suke; Yuasa, Shinsuke; Li, Weizhen; Yamakawa, Hiroyuki; Tanaka, Tomofumi; Onitsuka, Takeshi; Shimoji, Kenichiro; Ohno, Yohei; Egashira, Toru; Kaneda, Ruri; Murata, Mitsushige; Hidaka, Kyoko; Morisaki, Takayuki; Sasaki, Erika; Suzuki, Takeshi; Sano, Motoaki
- Abstract
Several applications of pluripotent stem cell (PSC)-derived cardiomyocytes require elimination of undifferentiated cells. A major limitation for cardiomyocyte purification is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamine methyl ester perchlorate, could be used to selectively mark embryonic and neonatal rat cardiomyocytes, as well as mouse, marmoset and human PSC-derived cardiomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardiomyocytes transplanted into testes did not induce teratoma formation. Moreover, aggregate formation of PSC-derived cardiomyocytes through homophilic cell-cell adhesion improved their survival in the immunodeficient mouse heart. Our approaches will aid in the future success of using PSC-derived cardiomyocytes for basic and clinical applications.
- Subjects
STEM cells; HEART cells; MITOCHONDRIA; MARMOSETS; FLUORESCENCE; TERATOMA; TESTIS tumors
- Publication
Nature Methods, 2010, Vol 7, Issue 1, p61
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1403