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- Title
A BioID-Derived Proximity Interactome for SARS-CoV-2 Proteins.
- Authors
May, Danielle G.; Martin-Sancho, Laura; Anschau, Valesca; Liu, Sophie; Chrisopulos, Rachel J.; Scott, Kelsey L.; Halfmann, Charles T.; Díaz Peña, Ramon; Pratt, Dexter; Campos, Alexandre R.; Roux, Kyle J.
- Abstract
The novel coronavirus SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic and has caused a major health and economic burden worldwide. Understanding how SARS-CoV-2 viral proteins behave in host cells can reveal underlying mechanisms of pathogenesis and assist in development of antiviral therapies. Here, the cellular impact of expressing SARS-CoV-2 viral proteins was studied by global proteomic analysis, and proximity biotinylation (BioID) was used to map the SARS-CoV-2 virus–host interactome in human lung cancer-derived cells. Functional enrichment analyses revealed previously reported and unreported cellular pathways that are associated with SARS-CoV-2 proteins. We have established a website to host the proteomic data to allow for public access and continued analysis of host–viral protein associations and whole-cell proteomes of cells expressing the viral–BioID fusion proteins. Furthermore, we identified 66 high-confidence interactions by comparing this study with previous reports, providing a strong foundation for future follow-up studies. Finally, we cross-referenced candidate interactors with the CLUE drug library to identify potential therapeutics for drug-repurposing efforts. Collectively, these studies provide a valuable resource to uncover novel SARS-CoV-2 biology and inform development of antivirals.
- Subjects
SARS-CoV-2; CHIMERIC proteins; COVID-19 pandemic; DEVELOPMENTAL biology; WEB hosting
- Publication
Viruses (1999-4915), 2022, Vol 14, Issue 3, p611
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v14030611