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- Title
Microbial conversion of an oil containing α-linolenic acid to an oil containing eicosapentaenoic acid.
- Authors
Shimizu, Sakayu; Kawashima, Hiroshi; Akimoto, Kengo; Shinmen, Yoshifumi; Yamada, Hideaki
- Abstract
Mycelia of arachidonic acid-producing fungi belonging to the genus Mortierella were found to convert an oil containing α-linolenic acid to an oil containing 5,8,11,14,17- cis-eicosapentaenoic acid (EPA). This conversion was observed when they were grown in a medium containing the oil, glucose and yeast extract at 28 C. On the screening of various oils, linseed oil, in which α-linolenic acid amounts to about 60% of the total fatty acids, was found to be the most suitable for EPA production. Under the optimal culture conditions, a selected strain, Mortierella alpina 20-17, converted 5.1% of the α-linolenic acid in the added oil into EPA, the EPA production reaching 1.35 g/l of culture broth (41.5 mg/g dry mycelia). This value corresponded to 7.1% (by weight) of the total fatty acids in the extracted lipids. The lipid was also found to be rich in arachidonic acid (12.3%). Other major fatty acids in the lipid were palmitic acid (4.4%), stearic acid (3.2%), oleic acid (13.5%), linoleic acid (13.7%), α-linolenic acid (38.5%) and γ-linolenic acid (0.9%).
- Publication
Journal of the American Oil Chemists' Society (JAOCS), 1989, Vol 66, Issue 3, p342
- ISSN
0003-021X
- Publication type
Article
- DOI
10.1007/BF02653286