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- Title
Isolation, Purification, Fractionation, and Hepatoprotective Activity of Polygonatum Polysaccharides.
- Authors
Wang, Yutong; Niu, Hongmei; Ma, Yue; Yuan, Guangxin
- Abstract
In this study, three homogeneous fractions, PSP-N-b-1, PSP-N-b-2, and PSP-N-c-1, were obtained from an aqueous extract of Polygonatum using DEAE cellulose column chromatography, CL-6B agarose gel chromatography, and Sephadex G100 chromatography. Their monosaccharide compositions and molecular weights were analyzed. The results revealed that PSP-N-b-1, PSP-N-b-2, and PSP-N-c-1 are primarily composed of six monosaccharides: Man (mannose), GlcA (glucuronic acid), Rha (rhamnose), GalA (galacturonic acid), Glc (glucose), and Ara (arabinose), with molecular weights of 6.3 KDa, 5.78 KDa, and 3.45 KDa, respectively. Furthermore, we observed that Polygonatum polysaccharides exhibited protective effects against CCL4-induced liver damage in HepG2 cells in vitro, operating through both anti-oxidant and anti-inflammatory mechanisms. Our research findings suggest that Polygonatum polysaccharides may emerge as a promising option in the development of hepatoprotective drugs or functional foods with anti-inflammatory and antioxidant properties.
- Subjects
POLYSACCHARIDES; MONOSACCHARIDES; GALACTURONIC acid; COLUMN chromatography; GLUCURONIC acid; MANNOSE; AGAROSE
- Publication
Molecules, 2024, Vol 29, Issue 5, p1038
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules29051038