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- Title
Isolation and characterization of racemase from Ensifer sp. 23-3 that acts on α-aminolactams and α-amino acid amides.
- Authors
Matsui, Daisuke; Fuhshuku, Ken-ichi; Nagamori, Shingo; Takata, Momoko; Asano, Yasuhisa
- Abstract
Limited information is available on α-amino-ε-caprolactam (ACL) racemase (ACLR), a pyridoxal 5′-phosphate-dependent enzyme that acts on ACL and α-amino acid amides. In the present study, eight bacterial strains with the ability to racemize α-amino-ε-caprolactam were isolated and one of them was identified as Ensifer sp. strain 23-3. The gene for ACLR from Ensifer sp. 23-3 was cloned and expressed in Escherichia coli. The recombinant ACLR was then purified to homogeneity from the E. coli transformant harboring the ACLR gene from Ensifer sp. 23-3, and its properties were characterized. This enzyme acted not only on ACL but also on α-amino-δ-valerolactam, α-amino-ω-octalactam, α-aminobutyric acid amide, and alanine amide.
- Subjects
AMINO acid amides; CAPROLACTAM; RACEMASES; RACEMIZATION; NITROGEN-fixing bacteria
- Publication
Journal of Industrial Microbiology & Biotechnology, 2017, Vol 44, Issue 11, p1503
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-017-1981-5