We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Target-responsive ratiometric fluorescent aptasensor for OTA based on energy transfer between [Ru(bpy)3]2+ and silica quantum dots.
- Authors
Zhu, Xi; Li, Wenjing; Lin, Liping; Huang, Xiaojuan; Xu, Huifeng; Yang, Guidi; Lin, Zhenyu
- Abstract
A ratiometric fluorescent aptasensor based on energy transfer between [Ru(bpy)3]2+ and silica quantum dots (silica QDs) for assaying OTA was fabricated. The aptamer for OTA was used as the gate to shield the fluorescent reagent [Ru(bpy)3]2+ into mesoporous silica nanoparticle (MSN). In the presence of OTA, the constrained [Ru(bpy)3]2+ was released from MSN due to a target-induced aptamer conformational change. The released [Ru(bpy)3]2+ adsorbed onto the negatively charged silica QDs through electrostatic interaction. This creates appearance of fluorescence from [Ru(bpy)3]2+ at 625 nm and decrease of the fluorescence from silica QDs at 442 nm owing to the energy transfer. The value of FL625nm/FL442nm was in proportion to the concentration of OTA in the range 0.5~100 ng mL−1 with a LOD of 0.08 ng mL−1. Practical applicability of this method was validated by the determination of OTA in flour samples.
- Subjects
QUANTUM dots; ENERGY transfer; SILICA; MESOPOROUS silica; ELECTROSTATIC interaction
- Publication
Microchimica Acta, 2020, Vol 187, Issue 5, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-020-04245-3