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- Title
DEVELOPMENT AND VALIDATION OF A FAST AND SIMPLE RP-HPLC METHOD FOR THE DETERMINATION OF DIOSMIN AND HESPERIDIN IN COMBINED TABLET DOSAGE FORM.
- Authors
Piponski, Marjan; Stoimenova, Tanja Bakovska; Topkoska, Marina; Stefov, Stefan; Piponska, Magdalena; Serafimovska, Gordana Trendovska
- Abstract
A fast, simple, accurate and robust reversed phase HPLC method for the simultaneous determination of two flavonoids, hesperidin and diosmin, in combined tablets was developed and validated. This method uses a short C8 column with dimensions of 75 mm × 4 mm with 5 μm particles thermostated at 30 °C, and a mobile phase composed of formic acid (pH 4.1 and 0.05%, V/V) and methanol (58:42, V/V), delivered at a flow rate of 1.2 ml/min, with UV detector signal monitoring at 280 nm and an injection volume of 5 μl. These chromatographic conditions yielded chromatograms with symmetric peaks of hesperidin, eluting at a ~2 min retention time, and diosmin, at a ~4.5 min retention time, with a total run time cycle of 6 min. The method validation parameters confirmed excellent values for accuracy, linearity and reproducibility. This method is suitable for routine analysis in pharmaceutical and food quality control laboratories.
- Subjects
HIGH performance liquid chromatography; DIOSMIN; HESPERIDIN; FLAVONOIDS; FORMIC acid
- Publication
Macedonian Journal of Chemistry & Chemical Engineering, 2018, Vol 37, Issue 2, p1
- ISSN
1857-5552
- Publication type
Article
- DOI
10.20450/mjcce.2018.1448